实验室：显微成像平台

职位：技术专家

工作职责

1）开展电子显微镜生物样品制备优化工作，主要负责常规生物样品、高压冷冻样品、免疫金标记样品和三维生物电镜样品制备方案设计和优化，提升平台制样服务水平；

2）负责平台120kV透射电镜日常维护、开放管理和用户培训工作，为用户提供测试服务和技术支撑；

3）负责实验室试剂耗材采购管理和实验室卫生安全等工作。

联系方式

西湖大学（云谷校区）E8-B124

zhenglimin@westlake.edu.cn

教育背景

2008/09-2014/12，福建农林大学，植物病理学，博士

2004/09-2008/06，陕西师范大学，生物技术，本科

工作经历

2021/09-至今，西湖大学，生物医学实验技术中心，技术专家

2015/01-2021/08，湖南省农业科学院

2019/05-2020/02，美国俄亥俄州立大学

技术成果

荣获2024年第三届“信立方杯”高校分析测试技术培训微课大赛三等奖。曾主持国家重点研发计划（政府间国际科技创新合作重点专项）、国家自然科学基金面上项目和青年基金等课题，发表论文多篇，参编专著2本。

部分发表文章

1）Ren R^#, Zheng L^#, Han J, Perdoncini Carvalho C, Miyashita S, Zhang D*, Qu F*. Intracellular bottlenecking permits no more than three tomato yellow leaf curl virus genomes to initiate replication in a single cell. PLoS Pathogens. 2023, 19(5): e1011365.

2）Chen J, Zheng L*, Shi X, Zhang S, Tan X, Zhao X, Lu B, Ye Q, Miao S, Liu Y*, Zhang D*. The nonstructural protein NSs encoded by tomato zonate spot virus suppresses RNA silencing by interacting with NbSGS3. Molecular Plant Pathology, 2022, 23(5): 707–719.

3）Chen Y, Zheng X, Wei H, Chen YD, Zheng KY, Mu Y, Zhao XY, Zhao LH, Gao YL, Zheng L*, Zhang J*. A plant virus mediates interspecific competition between its insect vectors in Capsicuum annuum. Journal of Pest Science, 2021, 94, 17-28.

4）Chen Q^#, Zheng L^#, Mao Q, Liu J, Wang H, Jia D, Chen H, Wu W, Wei T*. Fibrillar structures induced by a plant reovirus target mitochondria to activate typical apoptotic response and promote viral infection in insect vectors. PLoS Pathogens. 2019, 15(1): e1007510.

5）Chen Q^#, Zheng L^#, Zhong P, Jia D, Liu Y, Mao Q, Wei T*. Gelsolin of insect vectors negatively regulates actin-based tubule motility of plant reoviruses. Phytopathology Research. 2019, 1:19.

6）Zheng L, Chen H, Liu H, Xie L, Wei T*. Assembly of viroplasms by viral nonstructural protein Pns9 is essential for persistent infection of rice gall dwarf virus in its insect vector. Virus Research. 2015, 196:162-169.

7）Zheng L, Mao Q, Xie L, Wei T*. Infection route of rice grassy stunt virus, a tenuivirus, in the body of its brown planthopper vector, Nilaparvata lugens (Hemiptera: Delphacidae) after ingestion of virus. Virus Research. 2014, 188:170-173.