实验室：质谱与代谢平台
职位：主管
西湖大学（云栖校区）1号楼120室
13717644085/0571-85273907
fengshan@westlake.edu.cn
教育背景
2010/08-2014/07 清华大学生命科学学院 生物学 博士
2004/08-2007/07 清华大学生物科学与技术系 生物学 硕士
2000/09-2004/07 清华大学生物科学与技术系 生物学 学士
工作经历
2007/08-2010/07 北京教育学院生化环境系 助教/讲师
2014/07-2018/06 清华大学生命科学学院博士后
2016/11-2018/02 圣母大学生物科学系高级科研助理
2018/07-至今 西湖大学生物医学实验技术中心质谱与代谢平台 主管
工作职责
负责质谱与代谢平台的规划、建设和管理；
负责质谱与代谢平台设备的搭建与维护；
协助用户科学实验，为样品制备、数据分析等提供服务；
根据课题需求对现有实验方法进行优化，提供技术支持；
开展特色方法的自主研发工作。
技术成果
发表文章
(1)Shan Feng*, Xiaofei Wen, Xin Lu*, (2019) Nitropeptide Profiling and Identification Illustrated by Angiotensin II. J. Vis. Exp., 148: e59391. (* co-corresponding author)
(2) Lin Zhang, Hao Shen, Yiyi Gong, Xiaojing Pang, Meiqi Yi, Lin Guo, Jin Li, Sam Arroyo, Xin Lu, Sergey Ovchinnikov, Gong Cheng, Xudong Liu, Xu Jiang,Shan Feng* and Haiteng Deng*, (2019) Development of a dual-functional conjugate of antigenic peptide and Fc-III mimetics (DCAF) for targeted antibody blocking. Chem. Sci., 10: 3271-3280. (* co-corresponding author)
(3)Shan Feng, Xi Cheng, Lin Zhang, Xuemin Lu, Seema Chaudhary, Ruifang Teng, Christian Frederickson, Matthew Champion, Ren Zhao, Liang Cheng, Yiyi Gong, Haiteng Deng, and Xin Lu. (2018) Myeloid-Derived Suppressor Cells Inhibit T Cell Activation through Nitrating LCK in Mouse Cancers. PNAS, 115: 10094-10099.
(4) Yiyi Gong, Lin Zhang, Jin Li,Shan Feng* and Haiteng Deng*. (2016) Development of the Double Cyclic Peptide Ligand for Antibody Purification and Protein Detection. Bioconjug. Chem., 27: 1569-1573. (* co-corresponding author)
(5)Shan Feng#,Yuling Chen#, Fan Yang, Lei Zhang, Gulishana Adilijiang, Yan Gao and Haiteng Deng. (2015) Development of a Clickable Probe for Profiling of Protein Glutathionylation in the Central Cellular Metabolism of E. coli and Drosophila. Chem Biol, 22: 1461-1469. (# co-first author)
(6)Shan Feng, Lei Zhang, Gulishana Adilijiang, Jieyuan Liu, Minkui Luo and Haiteng Deng. (2014) Substrate profiling of glutathione S-transferase with engineered enzymes and matched glutathione analogues. Angew Chem Int Edit, 53: 7149-7153.
(7)Shan Feng#, Xiaoyan Zheng#, Dong Wang, Yiyi Gong and Haiteng Deng. (2014) Systematic analysis of reactivities and fragmentation of glutathione and its isomer GluCysGly. J Phys Chem A, 118: 8222-8228. (# co-first author)
(8)Shan Feng, Yiyi Gong, Gulishana Adilijiang and Haiteng Deng. (2013) Effects of the Fc-III Tag on Activity and Stability of Green Fluorescent Protein and Human Muscle Creatine Kinase. Protein Science, 22: 1008-1015.
(9)Shan Feng, Enbing Tian, Lei Zhang, Qingtao Wang and Haiteng Deng. (2012) Development of the Fc-III Tagged Protein Expression System for Protein Purification and Detection. PLOS ONE, 7 (8): e44208.
(10)Shan Feng, Zhen Xu and Yong-Bin Yan. (2008) Blocking creatine kinase refolding by trace amounts of copper ions. Journal of Inorganic Biochemistry, 102: 928-935.
(11)Shan Fengand Yong-Bin Yan. (2008) Effects of glycerol on the compaction and stability of the wild type and mutated rabbit muscle creatine kinase. Proteins: Structure, Function, and Bioinformatics, 71: 844-854.
(12)Shan Feng,Tong-Jin Zhao, Hai-Meng Zhou and Yong-Bin Yan. (2007)Effects of the single point genetic mutation D54G on muscle creatine kinase activity, structure and stability. TheInternational Journal of Biochemistry& Cell Biology, 39: 392-401.
