各位老师、同学：

大家好！

微量热泳动技术，即MicroScale Thermophoresis，简称MST技术。Monolith系列仪器采用MST技术定量分析分子间相互作用。对其分子中的一个分子进行荧光标记，使之成为报告分子，因此很容易探测到由结合引起的MST信号变化。实验结果通过软件自动分析，从而精确地计算Kd值。分子在溶液中的热动规律与其分子大小、电荷和水化层性质密切相关，任一性质的改变都会引起其运动规律的变化。因此，MST技术具有极高的灵敏度和广泛的应用范围，可以检测任何生物分子、化合物、纳米材料等分子间的相互作用，应用范围包括离子、小分子、到高分子量的蛋白复合体等。

设备将于2025年10月22日进行理论与上机培训，因场地和时间受限，如确定参加请务必扫描下方二维码报名，以便我们安排工作。

当日具体安排如下：

理论培训时间：2025年10月22日14:00-15:10

理论培训地点：云栖校区1#215

上机培训时间：2025年10月22日15:15-16:30

上机培训地点：云栖校区1#224

Dear Faculty and Students,

MicroScale Thermophoresis (MST) is a powerful technique for quantitative analysis of molecular interactions. The Monolith series instruments utilize MST to measure binding affinities by fluorescently labeling one of the interacting molecules as a reporter. Binding-induced changes in the thermophoretic movement of the fluorescent molecule are detected and automatically analyzed by the accompanying software to accurately determine the dissociation constant (Kd).Since thermophoretic movement in solution depends on molecular size, charge, and hydration properties, any change in these parameters can alter the signal, giving MST exceptionally high sensitivity. The technique is broadly applicable to the study of interactions among a wide range of biomolecules, compounds, and nanomaterials—from small ions and small molecules to large macromolecular complexes.

We are pleased to announce that a theoretical and hands-on training session for the MST instrument will be held on October 22, 2025. Due to limited space and time, please register by scanning the QR code below and reply to this email to confirm your participation.

Training Schedule:

Theoretical Session:October 22, 2025, 14:00–15:10

Location:Room 215, Building 1, Yunqi Campus

Hands-on Session:October 22, 2025, 15:15–16:30

Location:Room 224, Building 1, Yunqi Campus

We look forward to your participation!